Composite
Part:BBa_K1952011:Design
Designed by: Jessica Zhao Group: iGEM16_Kingsborough_NY (2016-10-19)
Hydrazine Synthase subunit beta (Kust-2860) with LacZ reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 966
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Utilized DNA from construction of K1952001 together with other spacer DNA and the coding region of LacZ alpha (for Blue-White screening) in Gibson assembly. Interchangable with parts for K1952009 assembly.
Source
DNA synthesized by Integrated DNA technologies.
References
Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. Nature Vol 479 [doi:10.1038/nature10453]